Composite

Part:BBa_K2802003:Design

Designed by: Shamlan M. S. Reshamwala   Group: iGEM18_ICT-Mumbai   (2018-10-05)


P2 ogr - PO promoter - P2 ogr - RBS + RFP + d.Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1301
    Illegal AgeI site found at 1413
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K274371, BBa_I746350 and BBa_K518012 were joined together using 3A Assembly.

K2802003.jpeg

The 719 bp BBa_K2802002 (lane 1) and 828 bp BBa_K518012 parts were joined together to make 1555 bp BBa_K2802003 (lane 3). Parts were digested with EcoRI and PstI.

Source

BBa_K274371 + BBa_I746350 + BBa_K518012

References